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Comparative Study
. 1989 Dec;86(23):9159-62.
doi: 10.1073/pnas.86.23.9159.

Antibacterial peptides from pig intestine: isolation of a mammalian cecropin

J Y Lee  1 , A Boman, C X Sun, M Andersson, H Jörnvall, V Mutt, H G Boman
Affiliations
Comparative Study

Antibacterial peptides from pig intestine: isolation of a mammalian cecropin

J Y Lee et al. Proc Natl Acad Sci U S A. 1989 Dec.

Abstract

Pig small intestine was used as starting material for a batchwise isolation of a peptide fraction enriched in antibacterial activities against Escherichia coli (anti-Ec factor) and against Bacillus megaterium (anti-Bm factor). Separation and further purification were by different types of chromatography. Sequence analysis showed the anti-Bm factor to be apparently similar to vasoactive intestinal peptide. The anti-Ec factor was found to have a 31-residue sequence that was cecropin-like. It was named cecropin P1 and its structure was confirmed by solid-phase synthesis. Synthetic cecropin P1 with and without C-terminal amide was assayed on eight different bacteria. Mobility comparison between synthetic and natural cecropin P1 indicates that the natural peptide has a free C-terminal carboxyl group.

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References

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    1. Eur J Biochem. 1988 Mar 1;172(2):371-6 - PubMed
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    1. Proc Natl Acad Sci U S A. 1989 Jan;86(1):262-6 - PubMed

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