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. 2015 Jan 1;20(1):e88-93.
doi: 10.4317/medoral.19953.

In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin

Maria-Fernanda Solá-Ruiz  1 , Carolina Pérez-Martínez, José-Javier Martín-del-Llano, Carmen Carda-Batalla, Carlos Labaig-Rueda
Affiliations

In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin

Maria-Fernanda Solá-Ruiz et al. Med Oral Patol Oral Cir Bucal. .

Abstract

Objectives: To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application.

Study design: Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM). Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM.

Results: Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation.

Conclusions: 1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium. 2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture. 3. The addition of melatonin tends to favor osteoblast proliferation.

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Conflict of interest statement

Conflict of interest statement: The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
Image captured under fluorescence microscope (20x).(a) DAPI staining attaches to nuclei DNA allowing cells to be counted.(b) Eosin staining attaches to cell cytoplasm making it possible to observe different morphology patterns.
Figure 2
Figure 2
SEM microphotos (a) On treated disks (Group II) osteoblasts proliferated randomly over the surface. (b) On Group III machined disks, osteoblasts proliferated in lines following the lines generated by machining.
Figure 3
Figure 3
SEM microphotograph (1500x) of Group II disk (with its surface characteristics). Note the flattened morphology adopted by osteoblasts with cytoplasmic extensions and filopods in order to better adhere to the surface and spread over it.
See this image and copyright information in PMC

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