Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase

Abstract

In this study, we have investigated various adding times of dextranase to the dextransucrase system to reveal the synergistic process of dextransucrase and dextranase. Dextranase added into the dextransucrase-sucrose system at different times gave rise to different main dextran products. The results showed that dextranase added into sucrose system at the same time with dextransucrase synthesized low molecular weight (Mw) dextran targeted to 5 kDa, while dextranase added during the reaction process of dextransucrase directionally prepared dextran with medium Mw of 10 kDa and 20 kDa. Fourier-transform infrared (FTIR) spectroscopy and (1)H Nuclear magnetic resonance (NMR) exhibited that the synthesized oligodextrans were mainly composed of α-1,6-glycosidic linkages (dextran 6587 Da, 96.82%; dextran 22,521 Da, 96.03%) and low α-1,3-glycosidic branch. The research established the relationship between the synergistic catalysis of the double enzymatic system and the synthesis of oligodextrans with different Mw.

Keywords: Oligodextrans; Penicillium aculeatum dextranase; Recombinant dextransucrase; Structural analysis; Synergistic catalysis.