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. 1989 Sep;80(9):904-9.
doi: 10.1111/j.1349-7006.1989.tb01733.x.

Enhancement of cytotoxicity of cisplatin in vitro by recombinant human tumor necrosis factor and/or recombinant human interferon-alpha, -beta and -gamma

C M Kim  1 , W S Hong, J O Lee, T W Kang, Y W Kim, J K Song, T K Yun, C Y Kim
Affiliations

Enhancement of cytotoxicity of cisplatin in vitro by recombinant human tumor necrosis factor and/or recombinant human interferon-alpha, -beta and -gamma

C M Kim et al. Jpn J Cancer Res. 1989 Sep.

Abstract

This study was conducted to investigate the modulatory effects of recombinant human tumor necrosis factor (rH-TNF) and recombinant human interferon (rH-IFN)-alpha, -beta and -gamma, either alone or in combination, on the cytotoxicity of cisplatin, using MTT assay, against MKN-45 (human stomach adenocarcinoma). MKN-45 was resistant to rH-TNF even at doses up to 10(3) U/ml. rH-IFN-gamma inhibited the survival of MKN-45 dose-dependently, while rH-IFN-alpha and -beta did not inhibit the survival of MKN-45 even at the highest concentrations tested (10(4) U/ml). Combination of rH-TNF with rH-IFN-alpha, -beta or -gamma did not significantly inhibit the survival of MKN-45, except for a combination of 10 U/ml of rH-TNF and 10(3) U/ml of rH-IFN-gamma (P less than 0.05). Cisplatin inhibited the survival of MKN-45 dose-dependently. By the simultaneous combination of cisplatin with rH-TNF and/or rH-IFN-alpha, -beta or -gamma, cytotoxicity of cisplatin was enhanced and the combination effects were additive. The effects of rH-TNF and rH-IFN-alpha, -beta and -gamma on the modification of cytotoxicity of cisplatin were evaluated in terms of modification index (MI), demonstrating that rH-TNF, rH-IFN-alpha, -beta and -gamma all augmented the cytotoxicity of cisplatin: MI values at 10(3) U/ml of rH-IFN-alpha, -beta and -gamma were 1.4, 1.4 and 2.3, respectively; those at the same concentrations of rH-IFN-alpha, -beta and -gamma in the presence of 10 U/ml of rH-TNF were 3.6, 2.5 and 5.1, respectively. These results demonstrating that the cytotoxicity of cisplatin was enhanced by rH-TNF and/or rH-IFN-alpha, -beta or -gamma suggest that cancer may be more effectively treated with the combination of cisplatin with these biological response modifiers than with cisplatin alone.

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References

    1. ) Loehrer , P. J. and Einhorn , L. H.Diagnosis and treatment. Drugs five years later: cisplatin . Ann, Intern. Med. , 100 , 704 – 713 ( 1984. ). - PubMed
    1. ) Le Chevalier , T. , Smith , F. P. , Harter , W. K. and Schein , P. S.Chemotherapy and combined modality therapy for locally advanced and metastatic gastric carcinoma . Semin. Oncol. , 12 , 46 – 53 ( 1985. ). - PubMed
    1. ) Mihich , E.Future perspectives for biological response modifiers: a viewpoint . Semin. Oncol , 13 , 234 – 254 ( 1986. ). - PubMed
    1. ) Inoue , M. and Tan , Y. H.Enhancement of actinomycin D‐ and cis‐diamimnedichloroplatinum(II)‐induced killing of human fibroblasts by human β‐interferon . Cancer Res. , 43 , 5484 – 5488 ( 1983. ). - PubMed
    1. ) Welander , C. E. , Morgan , T. M. , Homesley , H. D. , Trotta , P. P. and Spiegel , R. J.Combined recombinant human interferon alpha2 and cytotoxic agents studied in a clonogenic assay . Int. J. Cancer , 35 , 721 – 729 ( 1985. ). - PubMed

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