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. 2014 Oct;202(1):33-6.
doi: 10.1016/j.tvjl.2014.07.018. Epub 2014 Jul 24.

Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA

Priscilla F Gerber  1 , Qiaoling Gong  1 , Yao-Wei Huang  2 , Chong Wang  1 , Derald Holtkamp  1 , Tanja Opriessnig  3
Affiliations

Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA

Priscilla F Gerber et al. Vet J. 2014 Oct.

Abstract

An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. In serum samples from experimentally infected pigs (n = 35), anti-IgG PEDV antibodies were detected as early as 7 days post-infection. In field serum samples (n = 239), the diagnostic sensitivity of the S1 ELISA was 100% and the diagnostic specificity was 94%. The S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples (n = 133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.

Keywords: Diagnosis; ELISA; Porcine epidemic diarrhea virus; S1 protein; Serology.

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Figures

Fig. 1
Fig. 1
Porcine epidemic diarrhea virus-S1-Fc fusion expression plasmid.
Fig. 2
Fig. 2
Distribution of anti-IgG porcine epidemic diarrhea virus (PEDV) ELISA results by sample classification (negative, positive) relative to the assay cut-off (full line; optical density, OD, value 0.3), considering field samples from farms with known positive or negative PEDV status (A) or using the immunofluorescence assay (IFA) as the reference method to classify positive and negative samples (B). Samples with OD values ranging from 0.2 (dotted line) to 0.3 were considered to be indeterminate.
Fig. 3
Fig. 3
Distribution of anti-IgG (A) and IgA (B) porcine epidemic diarrhea virus (PEDV) ELISA results on colostrum samples by sample classification (negative, positive) relative to the assay cut-off (full line) considering field samples from farms with known positive or negative PEDV status.
Fig. 4
Fig. 4
Kinetics of IgG antibody to porcine enteric diarrhea virus (PEDV) over time from individual experimentally infected pigs as determined by the S1 ELISA.
See this image and copyright information in PMC

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