Aspirin differentially affects thromboxane and prostacyclin production by trophoblast and villous core compartments of human placental villi

Abstract

Low-dose aspirin has been used as prophylactic treatment for preeclampsia and fetal growth retardation, but the physiologic mechanisms for the beneficial effect of this therapy are unknown. We studied the effects of aspirin on eicosanoid production by different compartments of normal term human placental villi. Duplicate parallel incubations (n = 7) of whole villi, villous core tissues, and trophoblast were established on Millicell platform dishes in minimum essential medium in the presence or absence of 1 x 10(-4) or 1 x 10(-5) mol/L aspirin. Production rates of thromboxane A2 and prostacyclin were estimated by radioimmunoassay of their stable hydrolysis products, thromboxane B2 and 6-keto-prostaglandin F1 alpha, respectively. Our results indicate that 1 x 10(-4) mol/L aspirin inhibits thromboxane production in whole villi and villous core tissues denuded of their trophoblast layer but not in isolated trophoblast cells. The same concentration of aspirin also inhibits prostacyclin production in the isolated villous core but not in whole villi and not in isolated trophoblast. We conclude that aspirin can selectively inhibit thromboxane production in whole placental villi and differentially affects thromboxane and prostacyclin production by the trophoblast and villous core compartments.