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. 2014:2014:924296.
doi: 10.1155/2014/924296. Epub 2014 Jul 22.

Peritoneal air exposure elicits an intestinal inflammation resulting in postoperative ileus

Shanjun Tan  1 , Wenkui Yu  1 , Zhiliang Lin  1 , Qiyi Chen  1 , Jialiang Shi  1 , Yi Dong  1 , Kaipeng Duan  1 , Xiaowu Bai  2 , Lin Xu  2 , Jieshou Li  1 , Ning Li  1
Affiliations

Peritoneal air exposure elicits an intestinal inflammation resulting in postoperative ileus

Shanjun Tan et al. Mediators Inflamm. 2014.

Abstract

Background: The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism.

Methods: Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC).

Results: Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC.

Conclusions: The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response.

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Figures

Figure 1
Figure 1
Gastrointestinal transit. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. The data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Figure 2
Figure 2
Serum concentrations of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-10 in each group. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Figure 3
Figure 3
Concentrations of TNF-α, IL-1β, IL-6, and IL-10 in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Figure 4
Figure 4
Relative mRNA expression of TNF-α, IL-1β, IL-6, and IL-10 in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Figure 5
Figure 5
MPO activity in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
Figure 6
Figure 6
The levels of MDA, SOD, GSH-Px, and T-AOC in the tissues of ileum and colon. CG: control group; SG: sham group; EG1, EG2, and EG3: exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. Data are expressed as mean ± SD, n = 8. *P < 0.05, versus the CG group.
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