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. 2014 Sep;109(6):820-3.
doi: 10.1590/0074-0276140108. Epub 2014 Aug 19.

Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil

Nayara Zuchi  1 , Letícia Borges da Silva Heinen  1 , Marcelo Adriano Mendes dos Santos  2 , Fernanda Carla Pereira  1 , Renata Dezengrini Slhessarenko  1
Affiliations

Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil

Nayara Zuchi et al. Mem Inst Oswaldo Cruz. 2014 Sep.

Abstract

Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil.

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Figures

Fig. 1
Fig. 1. : distribution of patients with acute febrile illness from the state of Mato Grosso (MT), Brazil, investigated for arbovirus during a dengue outbreak in 2012. Cities with patients positive for Mayaro virus (MAYV) RNA and/or dengue virus 4 (DENV-4) are identified. Brazilian states: Amazonas (AM), Mato Grosso do Sul (MS), Pará (PA), Rondônia (RO), Tocantins (TO).
Fig. 2
Fig. 2. : prevalence of Mayaro virus in febrile patients from municipalities of the state of Mato Grosso, Brazil, during a dengue outbreak in 2012, according to patient age (A) and gender (B).
See this image and copyright information in PMC

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