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. 2014 Oct;25(4):328-33.
doi: 10.3802/jgo.2014.25.4.328. Epub 2014 Jun 18.

Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model

Ali Akdemir  1 , Burak Zeybek  2 , Levent Akman  1 , Ahment Mete Ergenoglu  1 , Ahmet Ozgur Yeniel  1 , Oytun Erbas  3 , Altug Yavasoglu  4 , Mustafa Cosan Terek  1 , Dilek Taskiran  3
Affiliations

Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model

Ali Akdemir et al. J Gynecol Oncol. 2014 Oct.

Abstract

Objective: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment.

Methods: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 μg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4°C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30°C for hormone assays.

Results: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Müllerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group.

Conclusion: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.

Keywords: Anti-Müllerian hormone; Cisplatin; Granulocyte-colony stimulating factor; Ovarian follicle; Ovary.

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Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Histological evaluation of ovaries in control (A), cisplatin (CP)+physiological saline (PS) (B), and cisplatin+granulocyte-colony stimulating factor (G-CSF) group (C). Cisplatin+PS group showed stromal and follicular damage. G-CSF treatment significantly reduced cisplatin-induced changes in ovaries (H&E, ×20). CL, corpus luteum; pr, primary follicle; sf, secondary follicle; tf, tertiary follicle; v, vessel.
See this image and copyright information in PMC

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