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. 2014 Nov;52(11):4023-6.
doi: 10.1128/JCM.01682-14. Epub 2014 Aug 20.

Rapid time to results and high sensitivity of the CarbaNP test on early cultures

Lai-yang Lee  1 , Tony M Korman  2 , Maryza Graham  3
Affiliations

Rapid time to results and high sensitivity of the CarbaNP test on early cultures

Lai-yang Lee et al. J Clin Microbiol. 2014 Nov.

Abstract

For rapid results, we adapted the CarbaNP test by using 5-hour-old cultures; the sensitivity and specificity for detection of carbapenemase production were 100% for non-OXA-producing organisms. The sensitivity from early cultures was 94% for detection of carbapenemase production in Enterobacteriaceae strains. Utilizing younger cultures allows the test to be incorporated into a single day's workflow, facilitating timely patient care and antimicrobial stewardship.

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Figures

FIG 1
FIG 1
CarbaNP and carbapenemase PCR results for isolates suspected to harbor a carbapenemase, from 5-h-old HBA cultures. See Table 1 for details on carbapenemase PCR-positive isolates.
FIG 2
FIG 2
CarbaNP results for carbapenemase PCR-positive organisms from 5-hour-old and 1-day-old cultures using horse blood agar.
See this image and copyright information in PMC

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