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Comparative Study
. 2014 Nov;52(11):4030-2.
doi: 10.1128/JCM.02340-14. Epub 2014 Aug 20.

Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species

Barbara A Qurollo  1 , Dana Riggins  1 , Alaire Comyn  1 , Merone T Zewde  1 , Edward B Breitschwerdt  2
Affiliations
Comparative Study

Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species

Barbara A Qurollo et al. J Clin Microbiol. 2014 Nov.

Abstract

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

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References

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