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. 1989:253B:213-8.
doi: 10.1007/978-1-4684-5676-9_32.

Utilization of 2'-deoxynad for ADP-ribose transfer reactions

D B Wasson  1 , H Yamanaka, D A Carson
Affiliations

Utilization of 2'-deoxynad for ADP-ribose transfer reactions

D B Wasson et al. Adv Exp Med Biol. 1989.

Abstract

2'-deoxyNAD was examined as a substrate for both mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation reactions. 2'-deoxyNAD is a substrate for the diphtheria toxin-catalyzed mono(ADP-ribosyl)ation of elongation factor-2, inactivating its function to enhance protein synthesis. On the other hand, 2'-deoxyNAD is a poor substrate for poly(ADP-ribose) polymerase. 2'-deoxyNAD was not synthesized intracellularly from deoxyATP, even when deoxyATP content was markedly increased by incubation of cells with deoxyadenosine and an adenosine deaminase inhibitor. 2'-deoxyNAD, because of its specificity, could be a quite useful reagent for the investigation of cellular mono(ADP-ribosyl)ation reactions.

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