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. 2014 Jan;7(1):e8888.
doi: 10.5812/jjm.8888. Epub 2014 Jan 1.

Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran

Mohammad Yousef Alikhani  1 , Zahra Karimi Tabar  1 , Fatemeh Mihani  1 , Enayat Kalantar  2 , Pegman Karami  1 , Mahnaz Sadeghi  3 , Shiva Ahdi Khosroshahi  4 , Safar Farajnia  5
Affiliations

Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran

Mohammad Yousef Alikhani et al. Jundishapur J Microbiol. 2014 Jan.

Abstract

Background: Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide. Resistance of P. aeruginosa strains to the broad-spectrum cephalosporins may be caused by extended-spectrum β-lactamases (ESBLs).

Objectives: The aim of this study was to determine the antimicrobial resistance patterns and prevalence of PER-1 and VEB-1 type genes among ESBL producing strains of P. aeruginosa.

Material and methods: A total of 106 P. aeruginosa isolates were collected from two university hospitals in Hamadan, Iran, during a7-month study (2009). The antimicrobial susceptibility of isolates was determined by disc diffusion method and interpreted according to the clinical and laboratory standards institute (CLSI) recommendations. Production of ESBL was determined by combined disk test and presence of PER-1 and VEB-1 type ESBL genes was identified by PCR.

Results: The resistance against broad-spectrum cephalosporins and monobactames were: cefepime (97%), cefotaxime (92.5%) ceftazidime (51%), and aztreonam (27%). Ciprofloxacin (91.5%), imipenem (84.9%) and meropenem (82.1%) were the most effective anti-pseudomonas agents in this study. The results revealed that 88.7% of the isolates were multidrug resistant, 58.25% of those were ESBL positive. Sixteen (26.6%), 9 (15%) and 3 (5%) strains among ESBL-producing strains contained blaPER-1, blaVEB and blaPER-1-blaVEB, respectively.

Conclusions: This study highlighted the need to establish antimicrobial resistance surveillance networks for P. aeruginosa to determine the appropriate empirical treatment regimens. The high prevalence of multidrug resistance and production of ESBLs in P. aeruginosa isolates confirms the necessity of protocols considering these issues in the hospitals.

Keywords: Antimicrobial Drug Resistance; Beta-lactamase; Pseudomonas aeruginosa.

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Figures

Figure 1.
Figure 1.. PCR Amplification of blaPER-1 Gene
L: 100 bp DNA ladder, C-: negative control, C+: positive control, N: PER-1 negative strains, 1–3: PCR products from PER-1positive strains.
Figure 2.
Figure 2.. PCR Amplification of blaVEB-1 Gene
L: 100 bp DNA ladder, C-: negative control, C+: positive control, N: negative strain, 1–4: PCR products fromVEB-1 positive strains.
See this image and copyright information in PMC

References

    1. Pagani L, Mantengoli E, Migliavacca R, Nucleo E, Pollini S, Spalla M, et al. Multifocal detection of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum beta-lactamase in Northern Italy. J Clin Microbiol. 2004;42(6):2523–9. doi: 10.1128/JCM.42.6.2523-2529.2004. - DOI - PMC - PubMed
    1. Isturiz RE, Carbon C. Antibiotic use in developing countries. Infect Control Hosp Epidemiol. 2000;21(6):394–7. doi: 10.1086/501780. - DOI - PubMed
    1. Livermore DM, Brown DF. Detection of beta-lactamase-mediated resistance. J Antimicrob Chemother. 2001;48 Suppl 1:59–64. - PubMed
    1. Ben-Mahrez Kamel, Rejiba Samia, Belhadj Cherifa, Belhadj Omrane. β-lactamase-mediated resistance to extended spectrum cephalosporins among clinical isolates of Pseudomonas aeruginosa. Res Microbiol. 1999;150(6):403–406. doi: 10.1016/s0923-2508(99)80075-8. - DOI - PubMed
    1. Lee S, Park YJ, Kim M, Lee HK, Han K, Kang CS, et al. Prevalence of Ambler class A and D beta-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J Antimicrob Chemother. 2005;56(1):122–7. doi: 10.1093/jac/dki160. - DOI - PubMed

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