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. 2014:1192:229-37.
doi: 10.1007/978-1-4939-1173-8_17.

Identification of murine basophils by flow cytometry and histology

Christian Schwartz  1 , David Voehringer
Affiliations

Identification of murine basophils by flow cytometry and histology

Christian Schwartz et al. Methods Mol Biol. 2014.

Abstract

Here we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4(-)CD19(-)CD49b(+)IgE(+) cells in flow cytometric analysis. When IgE levels are low one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.

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