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. 2014:1196:49-57.
doi: 10.1007/978-1-4939-1242-1_4.

A genetic strategy to obtain P-Gal4 elements in the Drosophila Hox genes

Luis de Navas  1 , David Foronda, Delia Del Saz, Ernesto Sánchez-Herrero
Affiliations

A genetic strategy to obtain P-Gal4 elements in the Drosophila Hox genes

Luis de Navas et al. Methods Mol Biol. 2014.

Abstract

The Drosophila Gal4/UAS system allows the expression of any gene of interest in restricted domains. We devised a genetic strategy, based on the P-element replacement and UAS-y (+) techniques, to generate Gal4 lines inserted in Hox genes of Drosophila that are, at the same time, mutant for the resident genes. This makes possible to express different wild-type or mutant Hox proteins in the precise domains of Hox gene expression, and thus to test the functional value of these proteins in mutant rescue experiments.

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