Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:2014:471642.
doi: 10.1155/2014/471642. Epub 2014 Aug 6.

Corneal epithelial wound healing promoted by verbascoside-based liposomal eyedrops

Luigi Ambrosone  1 , Germano Guerra  2 , Mariapia Cinelli  3 , Mariaelena Filippelli  2 , Monica Mosca  1 , Francesco Vizzarri  4 , Dario Giorgio  2 , Ciro Costagliola  2
Affiliations

Corneal epithelial wound healing promoted by verbascoside-based liposomal eyedrops

Luigi Ambrosone et al. Biomed Res Int. 2014.

Abstract

Different liposomal formulations were prepared to identify those capable of forming eyedrops for corneal diseases. Liposomes with neutral or slightly positive surface charge interact very well with the cornea. Then these formulations were loaded with verbascoside to heal a burn of corneal epithelium induced by alkali. The cornea surface affected involved in wound was monitored as a function of time. Experimental results were modeled by balance equation between the rate of healing, due to the flow of phenylpropanoid, and growth of the wound. The results indicate a latency time of only three hours and furthermore the corneal epithelium heals in 48 hours. Thus, the topical administration of verbascoside appears to reduce the action time of cells, as verified by histochemical and immunofluorescence assays.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Morphological comparison between alkali burns in hares treated with verbascoside loaded liposomal eyedrops and hares treated only with liposomes, as a function of time.
Figure 2
Figure 2
Fraction of cornea burned as a function of time for hares treated with verbascoside loaded liposomal eyedrops (•) and hares treated only with liposomes (○).The curve is the fit of (5) to the experimental data.
Figure 3
Figure 3
H&E staining in hares treated with verbascoside loaded liposomal eyedrops and hares treated only with liposomes. After alkali burn corneal epithelium appears thinner than normal and with superficial layer completely removed ((a)–(c)). Epithelial layer shows a restored normal thickness after treatment (b) but in untreated animals appears still thinner (d). Original magnification ×20.
Figure 4
Figure 4
Representative images of nick end-incorporated nucleotides (for evaluation of apoptosis) immunofluorescent green staining in hares treated with verbascoside loaded liposomal eyedrops. Alkali burn induces in epithelial cell an increasing intensely green nuclear apoptotic staining (a) comparing normal nuclear staining with DAPI (b); merge image shows a plastic picture of this phenomenon (c). Verbascoside treatment shows a large reduction of intensely green apoptotic epithelial cells (d) with respect to nonapoptotic cells stained with DAPI (e); merge image represents clearly this condition (f). Original magnification ×20.
See this image and copyright information in PMC

References

    1. Costagliola C, Iuliano G, Menzione M, Nesti A, Simonelli F, Rinaldi E. Systemic human diseases as oxidative risk factors in cataractogenesis. I. Diabetes. Ophthalmic Research. 1988;20(5):308–316. - PubMed
    1. Cattaneo F, Iaccio A, Guerra G, Montagnani S, Ammendola R. NADPH-oxidase-dependent reactive oxygen species mediate EGFR transactivation by FPRL1 in WKYMVm-stimulated human lung cancer cells. Free Radical Biology and Medicine. 2011;51(6):1126–1136. - PubMed
    1. Dell'omo R, Semeraro F, Guerra G, et al. Short-time prone posturing is well-tolerated and reduces the rate of unintentional retinal displacement in elderly patients operated on for retinal detachment. BMC Surgery. 2013;13(supplement 2, article S55) - PMC - PubMed
    1. Conti V, Russomanno G, Corbi G, et al. Aerobic training workload affects human endothelial cells redox homeostasis. Medicine and Science in Sports and Exercise. 2013;45(4):644–653. - PubMed
    1. Haigis MC, Yankner BA. The Aging Stress Response. Molecular Cell. 2010;40(2):333–344. - PMC - PubMed

MeSH terms