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. 2014 Sep;11(9):899-901.
doi: 10.1038/nmeth.3073.

A critique of methods for temperature imaging in single cells

Guillaume Baffou  1 , Hervé Rigneault  1 , Didier Marguet  2 , Ludovic Jullien  3
Affiliations

A critique of methods for temperature imaging in single cells

Guillaume Baffou et al. Nat Methods. 2014 Sep.

Abstract

We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.

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