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Comment
. 2015 Mar 1;211(5):846-7.
doi: 10.1093/infdis/jiu494. Epub 2014 Sep 1.

Does β-toxin production contribute to the cytotoxicity of hypervirulent Staphylococcus aureus?

Céline Dupieux  1 , Caroline Camus  2 , Gérard Lina  1 , François Vandenesch  1 , Frédéric Laurent  1 , Jean-Philippe Rasigade  1
Affiliations
Comment

Does β-toxin production contribute to the cytotoxicity of hypervirulent Staphylococcus aureus?

Céline Dupieux et al. J Infect Dis. .
No abstract available

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Figures

Figure 1.
Figure 1.
The excision of the β-toxin–converting phage φSa3 is induced by oxidative stress but not by intracellular infection and does not enhance cytotoxicity in the hypervirulent S. aureus strain SF8300. A, The proportion of β-toxin-producing variants was significantly higher in strains SF8300 and SF8300Δhla after 3 hours of incubation in brain-heart infusion (BHI) broth with 1 mM H2O2, compared with BHI broth alone; however, the same result was not observed after 6 hours of intracellular infection of MG-63 osteoblastic cells, compared with Dulbecco's modified Eagle's medium (DMEM) alone. The results were obtained from 3 independent experiments. Approximately 300 colonies were analyzed after intracellular passage, and 3000 colonies were analyzed under the other conditions. Differences in proportions were tested for significance, using the Fisher exact test. Error bars indicate exact binomial 95% confidence intervals. B, β-toxin–producing variants of strains SF8300 and SF8300Δhla did not exhibit enhanced cytotoxicity in the intracellular infection model of MG-63 cells with a 24-hour incubation period. The cytotoxicity percentage was quantified using a lactate dehydrogenase–based assay, with uninfected cells and cells lysed by osmotic shock corresponding to 0% and 100% toxicity, respectively. The results were obtained from 3 independent experiments performed in triplicate. Differences between groups were tested using the Mann–Whitney U test. The significance threshold was set at .05 for all tests. *P < .05. Abbreviations: hla, α-toxin–encoding gene; hlb, β-toxin–encoding gene; NS, not significant.
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Comment in

  • Reply to Dupieux et al.
    Schlievert P, Salgado-Pabon W, Herrera A, Vu BG, Stach CS, Merriman JA. Schlievert P, et al. J Infect Dis. 2015 Mar 1;211(5):847-8. doi: 10.1093/infdis/jiu495. Epub 2014 Sep 1. J Infect Dis. 2015. PMID: 25180237 No abstract available.

Comment on

References

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