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. 2014 Jul;4(7):538-44.
doi: 10.12980/APJTB.4.2014C1168.

Antioxidant potential of Rumex vesicarius L.: in vitro approach

Tajdar Husain Khan  1 , Majid Ahmad Ganaie  1 , Nasir Ali Siddiqui  2 , Aftab Alam  3 , Mohd Nazam Ansari  1
Affiliations

Antioxidant potential of Rumex vesicarius L.: in vitro approach

Tajdar Husain Khan et al. Asian Pac J Trop Biomed. 2014 Jul.

Abstract

Objective: To assess in-vitro antioxidant activity of different fraction and perform high performance thin layer chromatography fingerprint analysis of most active fraction of Rumex vesicarius L. (R. vesicarius).

Methods: In the present study, acetone, ethyl acetate, n-butanol, and methanol extracts of R. vesicarius were evaluated for radical scavenging activity by studying the inhibition of the level of lipid peroxidation induced by Fe(++)/ascorbate, DNA sugar damage, scavenging of hydrogen peroxide, diphenylphosphine DPPH radical scavenging activity, total phenolic content, total flavonoids content and total proanthocyanidin. High performance thin layer chromatography finger print profiling of R. vesicarius L. was also done.

Results: Lipid peroxidation induced by the iron/ascorbate system, hydrogen peroxide, diphenylphosphine and DNA sugar damage were inhibited by the addition of different extract of R. vesicarius. Among them, methanolic extract showed maximum efficacy. The methanolic extract showed the highest total phenolic, total flavonoids and total proanthocyanidin contents.

Conclusions: The results suggest that the extracts can be a vital source of phytochemical antioxidants.

Keywords: DNA sugar damage; DPPH; HPTLC; Rumex vesicarius L.; antioxidant.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. Photo documentation of methanolic extract of R. vesicarius at 254 nm.
Figure 2.
Figure 2.. Photo documentation of methanolic extract of R. vesicarius at 366 nm.
See this image and copyright information in PMC

References

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