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. 1989 Aug;13(2):213-22.
doi: 10.1007/BF00016139.

Maize pyruvate decarboxylase mRNA is induced anaerobically

P M Kelley  1
Affiliations

Maize pyruvate decarboxylase mRNA is induced anaerobically

P M Kelley. Plant Mol Biol. 1989 Aug.

Abstract

A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

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