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Comment
. 2014 Sep 23;111(38):13682-3.
doi: 10.1073/pnas.1414484111. Epub 2014 Sep 5.

Toward in vitro models of brain structure and function

Michael L Shuler  1 , James J Hickman  2
Affiliations
Comment

Toward in vitro models of brain structure and function

Michael L Shuler et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
An example of the 3D culture of rat hippocampal neurons in a collagen-based hydrogel immunostained with microtubule-associated protein-2. The cells formed signature connections in 3D and had superior survival to 2D-only cultures.
See this image and copyright information in PMC

Comment on

  • Bioengineered functional brain-like cortical tissue.
    Tang-Schomer MD, White JD, Tien LW, Schmitt LI, Valentin TM, Graziano DJ, Hopkins AM, Omenetto FG, Haydon PG, Kaplan DL. Tang-Schomer MD, et al. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13811-6. doi: 10.1073/pnas.1324214111. Epub 2014 Aug 11. Proc Natl Acad Sci U S A. 2014. PMID: 25114234 Free PMC article.

References

    1. Faul M, Xu L, Wald MM, Coronado VG. 2010. Traumatic Brain Injury in the United States: Emergency Department Visits, Hospitalizations and Deaths 2002–2006 (National Center for Injury Prevention and Control, Centers for Disease Control and Prevention, Atlanta)
    1. Tang-Schomer MD, et al. Bioengineered functional brain-like cortical tissue. Proc Natl Acad Sci USA. 2014;111:13811–13816. - PMC - PubMed
    1. Morrison B, III, Elkin BS, Dollé JP, Yarmush ML. In vitro models of traumatic brain injury. Annu Rev Biomed Eng. 2011;13:91–126. - PubMed
    1. Frampton JP, Hynd MR, Shuler ML, Shain W. Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture. Biomed Mater. 2011;6(1):015002. - PubMed
    1. Odawara A, Gotch M, Suzuki I. A three-dimensional neuronal culture technique that controls the direction of neurite-elongation and the portion of soma to mimic the layered structure of the brain. RSC Adv. 2013;3(45):23620–23630.

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