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. 2014 Dec:107:1-7.
doi: 10.1016/j.mimet.2014.08.022. Epub 2014 Sep 3.

To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

Marisol Aguirre  1 , Javier Ramiro-Garcia  2 , Marjorie E Koenen  3 , Koen Venema  4
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To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

Marisol Aguirre et al. J Microbiol Methods. 2014 Dec.

Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.

Keywords: Fecal inoculum preparation; Fermentation; Gut microbiota; In vitro model; Pooling.

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