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. 2014 Oct;11(10):1045-8.
doi: 10.1038/nmeth.3101. Epub 2014 Sep 7.

A sentinel protein assay for simultaneously quantifying cellular processes

Martin Soste  1 , Rita Hrabakova  2 , Stefanie Wanka  3 , Andre Melnik  1 , Paul Boersema  1 , Alessio Maiolica  4 , Timon Wernas  1 , Marco Tognetti  1 , Christian von Mering  3 , Paola Picotti  1
Affiliations

A sentinel protein assay for simultaneously quantifying cellular processes

Martin Soste et al. Nat Methods. 2014 Oct.

Abstract

We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.

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