Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2014 Jul 15;7(8):5192-5.
eCollection 2014.

Successful off-label use of the Cepheid Xpert GBS in a late-onset neonatal meningitis by Streptococcus agalactiae

Vincenzo Savini  1 , Roberta Marrollo  1 , Eleonora Coclite  2 , Paola Fusilli  2 , Carmine D'Incecco  2 , Paolo Fazii  1
Affiliations
Case Reports

Successful off-label use of the Cepheid Xpert GBS in a late-onset neonatal meningitis by Streptococcus agalactiae

Vincenzo Savini et al. Int J Clin Exp Pathol. .

Abstract

We report the case of a late-onset neonatal meningitis by Streptococcus agalactiae (group B Streptococcus - GBS) that was diagnosed with a latex agglutination assay (on cerebrospinal fluid, CSF), as well as by using, for the first time, Xpert GBS (Cepheid, US) on CSF. Due to empirical antibiotics given before sampling, both CSF and blood culture were negative, so the abovementioned diagnostics was crucial. Moreover, the Xpert GBS assay, performed according to an off-label, modified protocol (the system is designed for GBS-carriage intrapartum screening, based on a completely automated real time-Polymerase Chain Reaction) quickly detected the organism's genome target. Although further investigation on this test's performace on CSF is required, then, we trust it may be a promising, quick and precise diagnostic method for infections in newborns.

Keywords: Cepheid; GBS; Lancefield group B; Streptococcus agalactiae; Xpert GBS; gestation; meningitis; pregnancy.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Raising of the GBS curve, indicating test positivity, after about 25 RT-PCR cycles.
See this image and copyright information in PMC

References

    1. Filleron A, Lombard F, Jacquot A, Jumas-Bilak E, Rodière M, Cambonie G, Marchandin H. Group B streptococci in milk and late neonatal infections: an analysis of cases in the literature. Arch Dis Child Fetal Neonatal. 2014;99:F41–7. - PubMed
    1. Jones CA. Maternal transmission of infectious pathogens in breast milk. J Paediatr Child Health. 2001;37:576–582. - PubMed
    1. Cox F, Taylor L. Adherence of group B streptococci to buccal epithelial cells in neonates with different gestational ages. J Perinat Med. 1990;18:455–458. - PubMed
    1. Butter MNW, de Moor CE. Streptococcus agalactiae as a cause of meningitis in the newborn, and of bacteraemia in adults. Antoine van Leeuwenhoek. 1967;33:439–450.
    1. Verani JR, McGee L, Schrag SJ Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC) Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC, 2010. MMWR Recomm Rep. 2010;59:11–36. - PubMed

Publication types

MeSH terms

LinkOut - more resources