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. 2013 Dec 31;9(3):956-9.
doi: 10.4056/sigs.4618288. eCollection 2014 Jun 15.

Non-contiguous finished genome sequence and description of Halopiger goleamassiliensis sp. nov

Hassani Imene Ikram  1 , Robert Catherine  2 , Michelle Caroline  2 , Raoult Didier  2 , Hacène Hocine  1 , Desnues Christelle  2
Affiliations

Non-contiguous finished genome sequence and description of Halopiger goleamassiliensis sp. nov

Hassani Imene Ikram et al. Stand Genomic Sci. .

Abstract

Halopiger goleamassiliensis strain IIH3(T) sp. nov. is a novel, extremely halophilic archaeon within the genus Halopiger. This strain was isolated from an evaporitic sediment in El Golea Lake, Ghardaïa region (Algeria). The type strain is strain IIH3(T). H. goleamassiliensis is moderately thermophilic, neutrophilic, non-motile and coccus-shaped. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,906,923 bp long genome contains 3,854 protein-encoding genes and 49 RNA genes (1 gene is 16S rRNA, 1 gene is 23S rRNA, 3 genes are 5S rRNA, and 44 are tRNA genes).

Keywords: Draft genome; Euryarchaeota; Extreme halophile; Halopiger goleamassiliensis; thermotolerant.

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Figures

Figure 1
Figure 1
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequence comparisons, showing the position of strain IIH3T and other related haloarchaeal species. GenBank accession numbers are indicated in parentheses. Sequences were aligned using MUSCLE, and phylogenetic inferences obtained using the MEGA software. Numbers at the nodes are from a bootstrap analysis done using 1,000 replicates to generate a majority consensus tree. Methanospirillum hungatei was used as outgroup.
Figure 2
Figure 2
Gram staining of Halopiger goleamassiliensis strain IIH3T.
Figure 3
Figure 3
Transmission electron microscopy of H. goleamassiliensis strain IIH3T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 1µm.
Figure 4
Figure 4
Reference mass spectrum from H. goleamassiliensis strain IIH3T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
Figure 5
Figure 5
Gel view comparing the H. goleamassiliensis strain IIH3T spectrum with those of other archaea. The Gel View displays the raw spectra of all loaded spectrum files arranged in a pseudo-gel like look. The x-axis records the m/z value. The left y-axis displays the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by the gray scale intensity. The scale shown on the right y-axis links the color to the peak intensity in arbitrary units.
Figure 6
Figure 6
Graphical circular map of the H. goleamassiliensis IIH3T genome. From the outside in: The first circle indicates the scaffolds, the next two circles show open reading frames oriented in the forward and reverse (colored by COG categories) directions, respectively. The fourth circle displays the rRNA gene operon (red) and tRNA genes (green). The fifth circle shows the G+C% content plot. The inner-most circle shows the GC skew, purple and olive indicating negative and positive values, respectively.
See this image and copyright information in PMC

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