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. 2014 Mar 15;9(3):775-82.
doi: 10.4056/sigs.4978673. eCollection 2014 Jun 15.

Draft genome sequence of Bacillus amyloliquefaciens HB-26

Xiao-Yan Liu  1 , Yong Min  1 , Kai-Mei Wang  1 , Zhong-Yi Wan  1 , Zhi-Gang Zhang  1 , Chun-Xia Cao  1 , Rong-Hua Zhou  1 , Ai-Bing Jiang  1 , Cui-Jun Liu  1 , Guang-Yang Zhang  1 , Xian-Liang Cheng  1 , Wei Zhang  2 , Zi-Wen Yang  1
Affiliations

Draft genome sequence of Bacillus amyloliquefaciens HB-26

Xiao-Yan Liu et al. Stand Genomic Sci. .

Abstract

Bacillus amyloliquefaciens HB-26, a Gram-positive bacterium was isolated from soil in China. SDS-PAGE analysis showed this strain secreted six major protein bands of 65, 60, 55, 34, 25 and 20 kDa. A bioassay of this strain reveals that it shows specific activity against P. brassicae and nematode. Here we describe the features of this organism, together with the draft genome sequence and annotation. The 3,989,358 bp long genome (39 contigs) contains 4,001 protein-coding genes and 80 RNA genes.

Keywords: Bacillus amyloliquefaciens HB-26; Plasmodiophora brassicae; The Next-Generation sequencing.

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Figures

Figure 1
Figure 1
General characteristics of B. amyloliquefaciens HB-26. (A) The colonial morphology pictures of strain HB-26. (B) Phase contrast micrograph of HB-26. (C) SDS-PAGE analysis of proteins of HB-26. Lane M, protein molecular weight marker; Lane 1, proteins of strain HB-26.
Figure 2
Figure 2
Neighbor-Joining Phylogenetic tree was generated using MEGA 4 based on 16S rRNA sequences. The strains and their corresponding GenBank accession numbers for 16S rDNA sequences are: A: B. amyloliquefaciens ML581 (KC692179.1); B: B. amyloliquefaciens JM-21 (KC752450.1); C: Bacillus strain HB-26 (HM138476); D: B. vallismortis WA3-7 (JF496475.1); E: B. sp.BYK1448 (HF549161.1); F: B. subtilis 2B (KF112078.1); G: B.methylotrophicus GZGL8 (JN999861.1); H: B.vallismortis D20 (KC441761.1); I: B.tequilensis L10 (JN700126.1); J: B. sp. C4(2013) (KC310834.1); K: B. subtilis WBZ (KC460988.1); L: B. Amyloliquefaciens CA81 (KF040978.1) ; M: B. sp. SWB30 (JX861886.1) ; N: B.methylotrophicus Ns7-22 (HQ831412.1); O: B. subtilis 26A (KC295415.1). The phylogenetic tree was constructed by using the neighbor-joining method within the MEGA software [30].
Figure 3
Figure 3
Graphical circular map of the Bacillus amyloliquefaciens HB-26 genome. From the outside to the center: genes on forward strand (color by COG categories), genes on reverse strand (color by COG categories), GC content, GC skew. The map was generated with the CGviewer server (Stothard Rearch Group: http://stothard.afns.ualberta.ca/cgview_server/).
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