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. 2014 Apr 10;9(3):1198-210.
doi: 10.4056/sigs.5501035. eCollection 2014 Jun 15.

Non-contiguous finished genome sequence and description of Anaerococcus provenciensis sp. nov

Affiliations

Non-contiguous finished genome sequence and description of Anaerococcus provenciensis sp. nov

Isabelle Pagnier et al. Stand Genomic Sci. .

Abstract

Anaerococcus provenciensis strain 9402080(T) sp. nov. is the type strain of A. provenciensis sp. nov., a new species within the genus Anaerococcus. This strain was isolated from a cervical abscess sample. A. provenciensis is a Gram-positive anaerobic cocci. Here, we describe the features of this organism, together with the complete genome sequence and annotation. The 2.26 Mbp long genome contains 2099 protein-coding and 57 RNA genes including 8 rRNA genes and exhibits a G+C content of 33.48%.

Keywords: Anaerococcus provenciensis; genome.

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Figures

Figure 1
Figure 1
Phylogenetic tree showing the position of Anaerococcus provenciensis strain 9402080T relative to other type strains within the genus Anaerococcus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum-likelihood method within the MEGA 4 software [34]. Numbers at the nodes are bootstrap values obtained from 500 replicates used to generate a majority consensus tree. Clostridium butyricum was used as the outgroup. The scale bar represents a 2% nucleotide sequence divergence.
Figure 2
Figure 2
Gram stain of A. provenciensis strain 9402080T
Figure 3
Figure 3
Transmission electron micrograph of A. provenciensis strain 9402080T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 1 µm.
Figure 4
Figure 4
Reference mass spectrum from A. provenciensis strain 9402080T. Spectra from 10 individual colonies were compared and a reference spectrum was generated.
Figure 5
Figure 5
A dendrogram based on the comparison of the A. provenciensis strain 9402080T MALDI-TOF reference spectrum with the spectra from 24 other species of the order of Clostridiales
Figure 6
Figure 6
Graphical circular map of the genome. From outside to the center: scaffolds are in grey (unordered), genes on forward strand (colored by COG categories), genes on reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, tm RNAs black, misc_RNA pink), GC content (black/grey), and GC skew (purple/olive).

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