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. 2015 May:97:78-86.
doi: 10.1016/j.steroids.2014.08.022. Epub 2014 Sep 6.

A new ELISA for quantification of brassinosteroids in plants

Andrey G Pradko  1 , Raisa P Litvinovskaya  2 , Alina L Sauchuk  2 , Svetlana V Drach  2 , Alexander V Baranovsky  2 , Vladimir N Zhabinskii  2 , Tatyana V Mirantsova  1 , Vladimir A Khripach  3
Affiliations

A new ELISA for quantification of brassinosteroids in plants

Andrey G Pradko et al. Steroids. 2015 May.

Abstract

Starting from (22R,23R)-2α,3α,22,23,26-pentahydroxy-5α-cholestan-6-one 26-hemisuccinate, conjugates of 28-norcastasterone with horse radish peroxidase and bovine serum albumin were prepared. The latter conjugate was injected into rabbits; produced polyclonal antibodies were used to quantitate 6-keto-brassinosteroids. The newly developed analytical system was used in combination with two other immunoenzymatic assays for brassinosteroids to determine individual compounds of this series. In addition, a direct method of brassinosteroid analysis was proposed. It has the advantage of requiring no sample pretreatment steps such as extraction with organic solvents and chromatography.

Keywords: Brassinosteroids; Flores Chamomillae; HPLC; Phytohormones; Synthesis; ЕLISA.

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