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. 2013 Mar 22;1(4):apps.1200313.
doi: 10.3732/apps.1200313. eCollection 2013 Apr.

Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae)

Yuzu Sakata  1 , Shingo Kaneko  2 , Azusa Hayano  3 , Miho Inoue-Murayama  3 , Takayuki Ohgushi  4 , Yuji Isagi  1
Affiliations

Isolation and characterization of microsatellite loci in the invasive herb Solidago altissima (Asteraceae)

Yuzu Sakata et al. Appl Plant Sci. .

Abstract

Premise of the study: Microsatellite markers were developed in the perennial herb Solidago altissima from populations within its introduced range in Japan to assess its population structure and to facilitate tracking of invasion expansion. •

Methods and results: Using 454 pyrosequencing, 16 microsatellite primer sets were developed for S. altissima. The primer sets were tested on 70 individuals sampled from three populations in Japan. The primers amplified di- and trinucleotide repeats with five to 25 alleles per locus, and the expected heterozygosity ranged from 0.46 to 0.92. •

Conclusions: These results indicate the utility of primers in S. altissima for future research on a wide range of applications, including tracking of invasion dynamics and investigating population genetics of the species.

Keywords: 454 sequencing; Asteraceae; Solidago altissima; invasion; polyploidy.

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