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. 2014 Feb 15;9(4):402-6.
doi: 10.4103/1673-5374.128245.

The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells

Ting Zou  1 , Xiangqi Tang  1 , Zhiling Huang  1 , Niangui Xu  1 , Zhiping Hu  1
Affiliations

The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells

Ting Zou et al. Neural Regen Res. .

Abstract

In this study, we established cell models for Parkinson's disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an MTT assay. These experimental findings indicate that the Pael-R gene has no role in the changes in rotenone-induced Parkinson's disease model cells.

Keywords: Pael-R; Parkinson's disease; RNA interference; apoptosis; mitochondria; nerve regeneration; neural regeneration; neurodegeneration; rotenone.

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Conflict of interest statement

Conflicts of interest: None declared.

Figures

Figure 1
Figure 1
Effect of RNA interference (RNAi) on Pael-R gene and protein expression in Parkinson's disease model cells. (A) Pael-R gene expression detected by reverse-transcription polymerase chain reaction (RT-PCR). (B) Pael-R protein expression detected by west-ern blot analysis. Expession of Pael-R gene and protein was decreased in Parkinson's disease model cells by RNA interference (aP < 0.05, vs. model group). Both expression of Pael-R gene and protein was almost the same in the control group and the model group, but was lower in the RNAi group. Data are expressed as absorbance ratio of target gene/protein to GAPDH (mean ± SD) and were analyzed using analysis of variance. M: Marker.
Figure 2
Figure 2
Effect of RNA interference (RNAi) on apoptosis and survival rate of Parkinson's disease model cells. (A–C) Morphological changes to apoptotic cells that appeared in model and RNAi groups were detected by Hoechst 33258 staining (fluorescence inverted microscope, ×400); apoptotic cells were stained as blue. (D) Apoptosis rate was detected by flow cytometry. (E) Cell survival rate was de-tected by MTT assay. The rate of apoptosis and survival rate in Parkinson's disease cells in the model group was not significantly different from that in the RNAi group. Data are expressed as mean ± SD and were analyzed using analysis of variance. aP < 0.05, vs. control group.
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