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Comparative Study
. 2014 Nov;52(11):4078-81.
doi: 10.1128/JCM.02048-14. Epub 2014 Sep 10.

Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke  1 , Enno Jacobs  2
Affiliations
Comparative Study

Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke et al. J Clin Microbiol. 2014 Nov.

Abstract

Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.

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