Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

doi:10.1128/JCM.02048-14

Comparative Study

. 2014 Nov;52(11):4078-81.

doi: 10.1128/JCM.02048-14. Epub 2014 Sep 10.

Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke¹, Enno Jacobs²

Affiliations

¹ TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany roger.dumke@tu-dresden.de.
² TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany.

PMID: 25210063
PMCID: PMC4313229
DOI: 10.1128/JCM.02048-14

Comparative Study

Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke et al. J Clin Microbiol. 2014 Nov.

. 2014 Nov;52(11):4078-81.

doi: 10.1128/JCM.02048-14. Epub 2014 Sep 10.

Authors

Roger Dumke¹, Enno Jacobs²

Affiliations

¹ TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany roger.dumke@tu-dresden.de.
² TU Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Dresden, Germany.

PMID: 25210063
PMCID: PMC4313229
DOI: 10.1128/JCM.02048-14

Abstract

Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.

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References

1. Waites KB, Talkington DF. 2004. Mycoplasma pneumoniae and its role as a human pathogen. Clin. Microbiol. Rev. 17:697–728. 10.1128/CMR.17.4.697-728.2004. - DOI - PMC - PubMed
1. Atkinson TP, Balish MF, Waites KB. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol. Rev. 32:956–973. 10.1111/j.1574-6976.2008.00129.x. - DOI - PubMed
1. Narita M. 2010. Pathogenesis of extrapulmonary manifestations of Mycoplasma pneumoniae infection with special reference to pneumonia. J. Infect. Chemother. 16:162–169. 10.1007/s10156-010-0044-X. - DOI - PubMed
1. Atkinson TP, Waites KB. 2014. Mycoplasma pneumoniae infections in childhood. Pediatr. Infect. Dis. J. 33:92–94. 10.1097/INF.0000000000000171. - DOI - PubMed
1. von Baum H, Welte T, Marre R, Suttorp N, Lück C, Ewig S. 2009. Mycoplasma pneumoniae pneumonia revisited within the German competence network for community-acquired pneumonia (CAPNETZ). BMC Infect. Dis. 9:62. 10.1186/1471-2334-9-62. - DOI - PMC - PubMed

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[1] Waites KB, Talkington DF. 2004. Mycoplasma pneumoniae and its role as a human pathogen. Clin. Microbiol. Rev. 17:697–728. 10.1128/CMR.17.4.697-728.2004. - DOI - PMC - PubMed

[2] Waites KB, Talkington DF. 2004. Mycoplasma pneumoniae and its role as a human pathogen. Clin. Microbiol. Rev. 17:697–728. 10.1128/CMR.17.4.697-728.2004. - DOI - PMC - PubMed

[3] Atkinson TP, Balish MF, Waites KB. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol. Rev. 32:956–973. 10.1111/j.1574-6976.2008.00129.x. - DOI - PubMed

[4] Atkinson TP, Balish MF, Waites KB. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol. Rev. 32:956–973. 10.1111/j.1574-6976.2008.00129.x. - DOI - PubMed

[5] Narita M. 2010. Pathogenesis of extrapulmonary manifestations of Mycoplasma pneumoniae infection with special reference to pneumonia. J. Infect. Chemother. 16:162–169. 10.1007/s10156-010-0044-X. - DOI - PubMed

[6] Narita M. 2010. Pathogenesis of extrapulmonary manifestations of Mycoplasma pneumoniae infection with special reference to pneumonia. J. Infect. Chemother. 16:162–169. 10.1007/s10156-010-0044-X. - DOI - PubMed

[7] Atkinson TP, Waites KB. 2014. Mycoplasma pneumoniae infections in childhood. Pediatr. Infect. Dis. J. 33:92–94. 10.1097/INF.0000000000000171. - DOI - PubMed

[8] Atkinson TP, Waites KB. 2014. Mycoplasma pneumoniae infections in childhood. Pediatr. Infect. Dis. J. 33:92–94. 10.1097/INF.0000000000000171. - DOI - PubMed

[9] von Baum H, Welte T, Marre R, Suttorp N, Lück C, Ewig S. 2009. Mycoplasma pneumoniae pneumonia revisited within the German competence network for community-acquired pneumonia (CAPNETZ). BMC Infect. Dis. 9:62. 10.1186/1471-2334-9-62. - DOI - PMC - PubMed

[10] von Baum H, Welte T, Marre R, Suttorp N, Lück C, Ewig S. 2009. Mycoplasma pneumoniae pneumonia revisited within the German competence network for community-acquired pneumonia (CAPNETZ). BMC Infect. Dis. 9:62. 10.1186/1471-2334-9-62. - DOI - PMC - PubMed

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Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

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Evaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae

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