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. 2014 Jul;10(39):359-66.
doi: 10.4103/0973-1296.137379.

Simultaneous determination of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β -D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography

Dongsheng Fan  1 , Yang Zhao  2 , Xin Zhou  2 , Xiaojian Gong  2 , Chao Zhao  2
Affiliations

Simultaneous determination of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β -D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography

Dongsheng Fan et al. Pharmacogn Mag. 2014 Jul.

Abstract

Objective: In the present study, a high performance liquid chromatography (HPLC) coupled with photodiode array detection was developed for simultaneous quantitation of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β-D- glucuronopyranoside methyl ester and quercetin in Polygonum perfoliatum L.

Materials and methods: The chromatographic separations were performed on a reversed-phase C18 column using a mobile phase composed of acetonitrile -0.5% aqueous acetic acid with gradient elution. The calibration curves for the analytes demonstrated good linearities within the investigated ranges. The satisfactory intra- and inter-day precision, repeatability and stability of the developed analytical method were shown in the method validation procedure. The recoveries of the established method ranged from 95.76 to 102.10% for all the analytes.

Results: This proposed method was successfully applied for simultaneous quantification of the four compounds in effective part of Polygonum perfoliatum L. from different regions. Hierarchical clustering analysis (HCA) and principal components analysis (PCA) were performed to characterize and classify the samples based on the contents of the four compounds in Polygonum perfoliatum L.

Conclusion: The established HPLC method combined with chemometric approaches was proven to be useful and efficient for quality control of Polygonum perfoliatum L.

Keywords: High performance liquid chromatography; Polygonum perfoliatum L.; diode array detector; hierarchical clustering analysis; principal components analysis; quality control.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
The structure of four compouds: (a) Esculetin; (b) Quercetin-3-O-β-D-glucuronide; (c) Quercetin-3-O-β-D-glucuronopyranoside methyl ester; (d) Quercetin
Figure 2
Figure 2
HPLC chromatograms of sample and standard of four compouds: (a) Esculetin; (b) Quercetin-3-O-β-D-glucuronide; (c) Quercetin-3-O-β-D-glucuronopyranoside methyl ester; (d) Quercetin
Figure 3
Figure 3
Dendrogram of the 38 tested samples from different locations
Figure 4
Figure 4
Scores Plot for the 38 tested samples from different locations based on quantitative data
Figure 5
Figure 5
PCA loading plots for PC1 and PC2
See this image and copyright information in PMC

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