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. 2014 Sep 11;9(9):e106596.
doi: 10.1371/journal.pone.0106596. eCollection 2014.

Interrelationships between ALOX5AP polymorphisms, serum leukotriene B4 level and risk of acute coronary syndrome

Guoping He  1 , Shan Ye  1 , Jingjiao Hui  1 , Dandan Shen  1 , Chuanping Qi  2 , Lianhong Xu  2 , Yichao Qian  1
Affiliations

Interrelationships between ALOX5AP polymorphisms, serum leukotriene B4 level and risk of acute coronary syndrome

Guoping He et al. PLoS One. .

Abstract

Background: We investigated the relationships between the ALOX5AP gene rs10507391 and rs4769874 polymorphisms, serum levels of leukotriene (LT) B4, and risk of acute coronary syndrome (ACS).

Methods: A total of 709 participants, comprising 508 ACS patients (ACS group) and 201 noncoronary artery disease patients with chest pain (control group) were recruited from the Han population of the Changwu region in China. Two polymorphic loci were genotyped using polymerase chain reaction and restriction fragment length polymorphism analysis. Serum LTB4 level was determined by enzyme-linked immunosorbent assay.

Results: Serum LTB4 levels were significantly higher (P<0.001) in the ACS group (median/interquartile range, 470.27/316.32 pg/ml) than in the control group (233.05/226.82 pg/ml). No statistical differences were observed between genotype, allele and haplotype frequencies for the tested loci in either the ACS group or the control group, even after adjustments were made for conventional risk factors by multivariate logistic regression. This suggests there is no association between the ALOX5AP rs10507391 and rs4769874 polymorphisms and ACS risk. Elevated serum LTB4 level was closely linked to ACS risk, and may be independent of traditional risk factors as a risk factor for ACS (P<0.001). There was no significant association between serum LTB4 levels and the two variants in either the ACS group or the control group.

Conclusions: Rs10507391, rs4769874 and its haplotypes in ALOX5AP are unrelated to ACS risk in the Chinese Han population of Changwu, but elevated serum LTB4 level is strongly associated with ACS risk. Serum LTB4 level is not subject to the influence of either the rs10507391, rs4769874 or the haplotype.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Determination of the rs10507391 genotype by PCR amplification and restriction analysis.
When the nucleotide A is present, a Vsp I restriction site is created. Lane 6: homozygous AA (187 bp and 25 bp). Lane 1,3,7,9–11: heterozygous AT (212 bp,187 bp and 25 bp). Lane 2,4,5,8,12: homozygous TT (212 bp). Lane M: DNA marker.
Figure 2
Figure 2. Determination of the rs4769874 genotype by PCR amplification and restriction analysis.
When the nucleotide G is present, an Bstu I restriction site is created. Lane 2–11: homozygous GG (380 bp and 229 bp). Lane1: heterozygous GA (609 bp,380 bp and 229 bp). Lane 12: homozygous AA (609 bp). Lane M: DNA marker.
Figure 3
Figure 3. Sequenced diagrams of the PCR amplification production of the rs10507391.
(A) A unimodal arrowed was homozygous AA. (B) A/T bimodal arrowed was heterozygous AT. (C) T unimodal arrowed was homozygous TT.
Figure 4
Figure 4. Sequenced diagrams of the PCR amplification production of the rs4769874.
(A) A unimodal arrowed was homozygous AA. (B) GA bimodal arrowed was heterozygous GA. (C) G unimodal arrowed was homozygous GG.
Figure 5
Figure 5. Plot box of serum LTB4 levels (pg/ml) between ACS and control groups.
Serum LTB4 level in ACS patients was significantly higher than those in controls (P value is less than 0.001).
Figure 6
Figure 6. Plot box of serum LTB4 levels (pg/ml) among ALOX5AP genotypes in ACS patients.
(A) Serum LTB4 level was similar among AA, AT and TT genotype of rs10507391. (B) Serum LTB4 level also was similar between (AA+GA) and GG genotype of rs4769874.
Figure 7
Figure 7. Plot box of serum LTB4 levels (pg/ml) between ALOX5AP haplotypes in ACS patients.
See this image and copyright information in PMC

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