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. 2015 Jun:114:146-54.
doi: 10.1016/j.phytochem.2014.08.007. Epub 2014 Sep 8.

A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

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A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

Juan Da et al. Phytochemistry. 2015 Jun.

Abstract

Ultra-performance liquid chromatography (UPLC) and Single Standard for Determination of Multi-Components (SSDMC) are becoming increasingly important for quality control of medicinal herbs; this approach was developed for Ganoderma lucidum. Special attention was necessary for the appropriate selection of markers, for determining the reproducibility of the relative retention times (RRT), and for the accuracy of conversion factors (F). Finally, ten components were determined, with ganoderic acid A serving as single standard. Stable system parameters were established, and with successful resolution of those issues, this analytical method could be used more broadly.

Keywords: Ganoderenic acids; Ganoderic acids; Ganoderma lucidum; Quantitative determination; Ultra performance liquid chromatography.

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