A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

Affiliations

¹ Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
² National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
³ National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
⁴ Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: wanyingwu@mail.shcnc.ac.cn.

PMID: 25212865
DOI: 10.1016/j.phytochem.2014.08.007

A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

Juan Da et al. Phytochemistry. 2015 Jun.

. 2015 Jun:114:146-54.

doi: 10.1016/j.phytochem.2014.08.007. Epub 2014 Sep 8.

Authors

Affiliations

¹ Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
² National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
³ National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
⁴ Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: wanyingwu@mail.shcnc.ac.cn.

PMID: 25212865
DOI: 10.1016/j.phytochem.2014.08.007

Abstract

Ultra-performance liquid chromatography (UPLC) and Single Standard for Determination of Multi-Components (SSDMC) are becoming increasingly important for quality control of medicinal herbs; this approach was developed for Ganoderma lucidum. Special attention was necessary for the appropriate selection of markers, for determining the reproducibility of the relative retention times (RRT), and for the accuracy of conversion factors (F). Finally, ten components were determined, with ganoderic acid A serving as single standard. Stable system parameters were established, and with successful resolution of those issues, this analytical method could be used more broadly.

Keywords: Ganoderenic acids; Ganoderic acids; Ganoderma lucidum; Quantitative determination; Ultra performance liquid chromatography.

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A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

Affiliations

A reproducible analytical system based on the multi-component analysis of triterpene acids in Ganoderma lucidum

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances