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. 2014:2014:135824.
doi: 10.1155/2014/135824. Epub 2014 Aug 6.

Green Synthesis of Silver Nanoparticles: Structural Features and In Vivo and In Vitro Therapeutic Effects against Helicobacter pylori Induced Gastritis

Muhammad Amin  1 , Sadaf Hameed  1 , Asghar Ali  1 , Farooq Anwar  2 , Shaukat Ali Shahid  3 , Imran Shakir  4 , Aqdas Yaqoob  1 , Sara Hasan  1 , Safyan Akram Khan  5 , Sajjad-Ur-Rahman  6
Affiliations

Green Synthesis of Silver Nanoparticles: Structural Features and In Vivo and In Vitro Therapeutic Effects against Helicobacter pylori Induced Gastritis

Muhammad Amin et al. Bioinorg Chem Appl. 2014.

Abstract

This study evaluates in vivo and in vitro anti-Helicobacter pylori (H. pylori) efficacy of silver nanoparticles (Ag-NPs) prepared via a cost-effective green chemistry route wherein Peganum harmala L. seeds extract was used as a reducing and capping agent. The structural features, as elucidated by surface plasmon resonance spectrophotometry, transmission electron microscopy, and powder X-ray diffraction spectroscopy, revealed the Ag-NPs synthesized to be polydispersed in nature and spherical in shape with 5-40 nm size. A typical Ag-NPs suspension (S5), with size being 15 nm, when tested in vitro against forty-two local isolates and two reference strains, showed a considerable anti-H. pylori activity. In case of in vivo trial against H. pylori induced gastritis, after oral administration of 16 mg/kg body weight of S5 for seven days, a complete clearance was recorded in male albino rates. In comparative time-killing kinetics, S5 exhibited dose- and time-dependent anti-H. pylori activity that was almost similar to tetracycline and clarithromycin, less than amoxicillin, but higher than metronidazole. Furthermore, S5 was found to be an equally effective anti-H. pylori agent at low (≤4) and high pH with no drug resistance observed even up to 10 repeated exposures while a significant drug resistance was recorded for most of the standard drugs employed. The present results revealed the potential of the synthesized Ag-NPs as safer bactericidal agents for the treatment of H. pylori induced gastritis.

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Figures

Figure 1
Figure 1
(a) Effect of PH-sus on the SPR of typical sample S5, (b) variation of the size of the silver-NPs with PH-sus using different techniques, SPR (♦), P-XRD (■), and TEM analysis (▲), (c) variation of SPR with temperature (S5), (d) effect of time on the synthesis of sample S5, and (e) stability of Ag-NPs versus time.
Figure 2
Figure 2
(a, e) TEM image and size distribution of S5, (b, f) TEM image and size distribution of S4, (c) TEM image of S3, (d) TEM image of S2, and (g) P-XRD pattern of S5 and SAED pattern of S5.
Figure 3
Figure 3
(a) FT-IR spectra of PH-sus and (b) FT-IR spectra of Ag-NPs (S5).
Figure 4
Figure 4
In vivo therapeutic efficacy of silver nanoparticles.
Figure 5
Figure 5
Time- and dose-dependent killing curves for (a) silver nanoparticles, (b) tetracycline, (c) metronidazole, and (d) amoxicillin, against H. pylori strain NCTC 11637.
Figure 6
Figure 6
Effect of medium pH on the anti-H. pylori activities of silver nanoparticles against NCTC 11637.
Figure 7
Figure 7
Resistance developments in H. pylori strain NCTC 11637 after repeated exposure to silver nanoparticles and standard antibiotics.
See this image and copyright information in PMC

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