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. 2014 Oct;32(10):1036-44.
doi: 10.1038/nbt.2999. Epub 2014 Sep 14.

Global analysis of protein structural changes in complex proteomes

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Global analysis of protein structural changes in complex proteomes

Yuehan Feng et al. Nat Biotechnol. 2014 Oct.

Abstract

Changes in protein conformation can affect protein function, but methods to probe these structural changes on a global scale in cells have been lacking. To enable large-scale analyses of protein conformational changes directly in their biological matrices, we present a method that couples limited proteolysis with a targeted proteomics workflow. Using our method, we assessed the structural features of more than 1,000 yeast proteins simultaneously and detected altered conformations for ~300 proteins upon a change of nutrients. We find that some branches of carbon metabolism are transcriptionally regulated whereas others are regulated by enzyme conformational changes. We detect structural changes in aggregation-prone proteins and show the functional relevance of one of these proteins to the metabolic switch. This approach enables probing of both subtle and pronounced structural changes of proteins on a large scale.

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References

    1. J Mol Biol. 2003 Nov 14;334(1):129-41 - PubMed
    1. Yeast. 1995 Jan;11(1):53-5 - PubMed
    1. Nat Biotechnol. 2012 Oct;30(10):918-20 - PubMed
    1. Arch Pharm (Weinheim). 2010 Apr;343(4):193-206 - PubMed
    1. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21984-9 - PubMed

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