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. 1997 Nov;119(46):11349-11350.
doi: 10.1021/ja972696a.

The S0 State of the Oxygen-Evolving Complex in Photosystem II Is Paramagnetic: Detection of an EPR Multiline Signal

Johannes Messinger  1 , John H Robblee  1 , Wa On Yu  1 , Kenneth Sauer  1 , Vittal K Yachandra  1 , Melvin P Klein  1
Affiliations

The S0 State of the Oxygen-Evolving Complex in Photosystem II Is Paramagnetic: Detection of an EPR Multiline Signal

Johannes Messinger et al. J Am Chem Soc. 1997 Nov.
No abstract available

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Figures

Figure 1
Figure 1
X-band EPR difference spectra from PS II membranes at 7 K: A, physiological S0 state minus S1 state in presence of methanol (1.5%, v/v), FCCP and PPBQ; the inset (4× amplified) was obtained after subtraction of the broad underlying signal; B, S2 state minus S1 with same additions, induced by 200 K continuous illumination (6 min); C, S0 state minus S1, both without methanol, but with PPBQ and FCCP addition; D, S0* state induced by NH2OH incubation in presence of 1.5% methanol (v/v) and 1 mM EDTA minus S1 with the same additions. For clarity, spectrum D is amplified by a factor of 3 relative to the other spectra; the asterisk at the spectrum labels a subtraction artifact that is probably due to different amounts of low-potential cytochrome b559+ in the S0* sample compared to the S1 control. The g = 2 regions containing the YDox radical signal were deleted for clarity. All measurements were recorded using a Varian E-109 X-band spectrometer with an E-102 microwave bridge and an Air Products helium flow cryostat. Instrument conditions: microwave power, 30 mW; modulation frequency, 100 kHz; modulation amplitude, 20 G; time constant, 0.5 s; scan time, 4 min.
Scheme 1
Scheme 1
Spin Coupling Scheme Used To Obtain the Spins S13 and S24 in the Vector Coupling Approach
See this image and copyright information in PMC

References

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