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. 2014 Jul 31:3:151.
doi: 10.4103/2277-9175.137839. eCollection 2014.

Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp

Jamshid Faghri  1 , Samane Bourbour  1 , Sharare Moghim  1 , Mohsen Meidani  2 , Hajiye Ghasemian Safaei  1 , Nafise Hosseini  1 , Bahram Nasr Esfahani  1 , Hussein Fazeli  1 , Mansour Sedighi  1
Affiliations

Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp

Jamshid Faghri et al. Adv Biomed Res. .

Abstract

Background: The aerobic Actinomycetes are a large group of soil-indwelling bacteria that are distributed in world-wide. These Gram-positive bacteria are most commonly associated with opportunistic infections in both immunocompromised and immunocompetent hosts.

Materials and methods: In this study, three phenotypic and deoxyribonucleic acid (DNA) extraction methods for isolation and identification of Nocardia genus were compared. Samples were taken in five different locations of Isfahan's suburb from hospitals area, parks, agricultural lands, gardens, arid lands with different soil temperature and pH.

Results: In this study, showed that slip-buried-method was better than two other phenotypic methods; 14 out of 70 soil samples (20%) were positive for Nocardia spp. DNA of positive samples were extracted with three techniques and DNA extraction by microwave technique was better than others. This technique was confirmed with observation of DNA bands on 1% agarose gel.

Conclusions: These bacteria are important in immune deficient patients such as cancer patients, transplant recipients, tuberculosis; acquired immunodeficiency syndrome etc., Their affluence is unsteady in different zones of the world. In this study, among the three phenotypic methods for the isolation of Nocardia slip-buried method was better than other methods. Among DNA extraction techniques, DNA extraction by microwave method would be selective method for DNA extraction of Nocardia spp. compared with others techniques.

Keywords: Deoxyribonucleic acid extraction techniques; Nocardia spp.; phenotypic methods; soil.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Chalky colonies of Nocardia
Figure 2
Figure 2
Wrinkled colonies of Nocardia
Figure 3
Figure 3
Partially acid-fast Nocardia organisms show reddish to purple filaments
Figure 4
Figure 4
Deoxyribonucleic acid extraction using the microwave oven technique (540 W at 13 s)
See this image and copyright information in PMC

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