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. 2014 Jun;32(3):239-41.

[Construction and identification of the Bifidobacterium expression system pGEX-TSOL18/B. longum of Taenia solium]

[Article in Chinese]
Bi-Ying Zhou, Mei-Chen Liu, Li-Fang He
  • PMID: 25223064

[Construction and identification of the Bifidobacterium expression system pGEX-TSOL18/B. longum of Taenia solium]

[Article in Chinese]
Bi-Ying Zhou et al. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2014 Jun.

Abstract

The TSOL18 gene of Taenia solium was synthesized and cloned into Escherichia coli-Bifidobacteria shuttle vector pGEX-1lambdaT. The recombinant plasmid pGEX-TSOL18 was transformed into Bifidobacterium longum with electroporation. The recombinant plasmid containing TSOL18 gene was identified by restriction endonuclease analysis, PCR and DNA sequencing. The length of synthesized TSOL18 gene was 393 bp. The results indicated that the Bifidobacteria expression system pGEX-TSOL18/B. longum was successfully constructed.

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