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. 2014 Sep;30(5):1022-6.
doi: 10.12669/pjms.305.4537.

Isolation and characterization of Human Mesenchymal Stromal Cells Derived from Placental Decidua Basalis; Umbilical cord Wharton's Jelly and Amniotic Membrane

Anahita Shaer  1 , Negar Azarpira  2 , Mahdokht H Aghdaie  3 , Elaheh Esfandiari  4
Affiliations

Isolation and characterization of Human Mesenchymal Stromal Cells Derived from Placental Decidua Basalis; Umbilical cord Wharton's Jelly and Amniotic Membrane

Anahita Shaer et al. Pak J Med Sci. 2014 Sep.

Abstract

Objective: Mesenchymal stromal cells (MSCs) are considered as an excellent source in regenerative medicine, but availability and ethical problems limited their routine use. Therefore, another available source with easy procedure and exempt from ethical debate is important. The purpose of this study is to isolate and characterize the MSCs from human placenta. The stromal cells were isolated from Placental Decidua Basalis (PDB-MSC), Umbilical cord Wharton's Jelly (WJ-MSC) and Amniotic Membrane (AM-MSC).

Methods: Full term human placentas (n=4), from cesarean section delivery were collected. Small fragments from different parts were cultures as explants. The immunophenotyping, mesodermal differentiation, growth kinetics and stemness gene expression was studied.

Results: The cultivated cells from three sources expressed CD44, CD105, and CD90. Gene expression of NANOG and OCT4 confirmed the undifferentiated state. The doubling-times for WJ-MSCs, PLC-MSCs and AM-MSCs, respectively, were 21±8h, 28±9h and 25±9h at passage three and 30±5h, 45±7h and 45±7h at passage tenth. The proliferative potential of WJ-MSCs tended to be higher than the other two sources.

Conclusion: The fetal derives stromal cells; especially the early passages of WJ-MSCs are available supplies for large scale production of MSC for using in clinical studies or research projects.

Keywords: Amnion; Mesenchymal stromal cells; Placenta; Umbilical cord.

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Figures

Fig.1
Fig.1
The photomicrographs were taken on day 10 - 14 of MSC in culture. The cells were grown from the edge of explants
Fig.2
Fig.2
The cells reached 85-90% confluency
Diagram.1
Diagram.1
Flow cytometry histogram of MSCs from three sources for mesenchymal stem cell (CD90, CD105, CD44), hematopoietic (CD34), and endothelial (CD133) markers. Purple lines indicate background fluorescence obtained with isotype control, Green histogram: signal for each specific antibody. Results represent at least 3 experiments
See this image and copyright information in PMC

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