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. 2014;24(6):1933-9.
doi: 10.3233/BME-141002.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector

Wenfang Zhao  1 , Kehai Liu  1 , Shunsheng Chen  1 , Man Man Zhu  1 , Hui Lv  1 , Jing Hu  1 , Yuan Mao  1
Affiliations

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector

Wenfang Zhao et al. Biomed Mater Eng. 2014.

Abstract

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

Keywords: Non-viral gene vector; P123; avß3; nuclear localization signal; polyethylenimine.

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