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. 2014 Dec 1:2:261-263.
doi: 10.1016/j.gdata.2014.08.004.

Gene Expression Profiling of Valvular Interstitial Cells in Rapacz Familial Hypercholesterolemic Swine

Ana M Porras  1 , Dhanansayan Shanmuganayagam  2 , Jennifer J Meudt  2 , Christian G Krueger  2 , Jess D Reed  2 , Kristyn S Masters  1
Affiliations

Gene Expression Profiling of Valvular Interstitial Cells in Rapacz Familial Hypercholesterolemic Swine

Ana M Porras et al. Genom Data. .

Abstract

Rapacz familial hypercholesterolemic (RFH) swine are a well-established model of human FH, a highly prevalent hereditary disease associated with increased risk of coronary artery disease and calcific aortic valve disease (CAVD). However, while these animals have been used extensively for the study of atherosclerosis, the heart valves from RFH swine have not previously been examined. We report the analysis of valvular interstitial cell gene expression in adult (two year old) and juvenile (three months old) RFH and WT swine by microarray analysis via the Affymetrix Porcine Genome Array (GEO #: GSE53997). Principal component and hierarchical clustering analysis revealed grouping and almost no variability between the RFH juvenile and WT juvenile groups. Additionally, only 21 genes were found differentially expressed between these two experimental groups whereas over 900 genes were differentially expressed when comparing either RFH or WT juvenile swine to RFH adults.

Keywords: familial hypercholesterolemia; microarray; swine; valve disease.

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Figures

Fig. 1
Fig. 1
Principal component plot of normalized expression values. The numbers in parentheses indicate the percentage of the variation captured by each principal component.
Fig. 2
Fig. 2
Hierarchical clustering of RFH and WT normalized microarray data based on the 30 genes with the highest variability across chips.
Fig. 3
Fig. 3
Histogram of the coefficients of variability for each probeset within (A) WT juvenile swine, (B) RFH juvenile swine, (C) RFH adult swine, and (D) all swine.
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