Proteomics analysis for finding serum markers of ovarian cancer

Abstract

A combination of peptide ligand library beads (PLLB) and 1D gel liquid chromatography-mass spectrometry/mass spectrometry (1DGel-LC-MS/MS) was employed to analyze serum samples from patients with ovarian cancer and from healthy controls. Proteomic analysis identified 1200 serum proteins, among which 57 proteins were upregulated and 10 were downregulated in the sera from cancer patients. Retinol binding protein 4 (RBP4) is highly upregulated in the ovarian cancer serum samples. ELISA was employed to measure plasma concentrations of RBP4 in 80 samples from ovarian cancer patients, healthy individuals, myoma patients, and patients with benign ovarian tumor, respectively. The plasma concentrations of RBP4 ranging from 76.91 to 120.08 ng/mL with the mean value 89.13 ± 1.67 ng/mL in ovarian cancer patients are significantly higher than those in healthy individuals (10.85 ± 2.38 ng/mL). Results were further confirmed with immunohistochemistry, demonstrating that RBP4 expression levels in normal ovarian tissue were lower than those in ovarian cancer tissues. Our results suggested that RBP4 is a potential biomarker for diagnostic of screening ovarian cancer.

Figure 1

1D-SDS-P1D-SDS-PAGE gel images of serum proteins after PLLB treatment. Lane 1: molecular weight marker. Lanes 2 and 3: serum proteins of healthy and ovarian cancer eluted from the PLLB.

Figure 2

Mean levels of RBP4 serum content measured with ELISA, showing that the expression of RBP4 in patients with ovarian cancer was significantly upregulated compared with the controls (P < 0.01).

Figure 3

Immunohistochemistry for RBP4. Tissues from ovarian cancer (a) and normal ovaries (b) were stained for RBP4. Original magnification ×10 (low power) or ×40 (high power).