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Case Reports
. 2014 Dec;52(12):4414-8.
doi: 10.1128/JCM.00967-14. Epub 2014 Sep 24.

Tenosynovitis caused by a novel nontuberculous Mycobacterium species initially misidentified as a member of the Mycobacterium tuberculosis complex

Patricia J Simner  1 , Emily P Hyle  2 , Seanne P Buckwalter  1 , John A Branda  3 , Barbara A Brown-Elliott  4 , Jameelah Franklin  5 , Nadege C Toney  6 , Tom J B de Man  6 , Richard J Wallace Jr  4 , Ravikiran Vasireddy  4 , Rajesh T Gandhi  7 , Nancy L Wengenack  8
Affiliations
Case Reports

Tenosynovitis caused by a novel nontuberculous Mycobacterium species initially misidentified as a member of the Mycobacterium tuberculosis complex

Patricia J Simner et al. J Clin Microbiol. 2014 Dec.

Abstract

We present a case of tenosynovitis caused by a novel, slowly growing, nonchromogenic, nontuberculous mycobacterium (NTM). Originally misidentified as Mycobacterium tuberculosis complex, the NTM cross-reacts with the M. tuberculosis complex nucleic acid hybridization probe, a M. tuberculosis gamma interferon release assay, and is closely related to M. tuberculosis by 16S rRNA gene sequencing.

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Figures

FIG 1
FIG 1
Image of a Middlebrook 7H10 plate demonstrating the colony morphology of the novel NTM that was consistent with M. tuberculosis morphology and that was described as a dry, wrinkled, flat, and crystalline nonchromogen.
FIG 2
FIG 2
Phylogenetic relationship of the novel NTM isolate (TBL 1200985) and related species of Mycobacterium based on 16S rRNA gene sequences. All sequences used, except for the novel NTM, are type strains. Sequences were aligned with MUSCLE (17) and the tree constructed by the neighbor-joining method using the Tamura-Nei distance model. All steps were performed using Geneious software. Mycobacterium abscessus CIP 104536 was used as an outgroup (GenBank accession no. AY457071). Branch support is recorded at the nodes as a percentage of 100 bootstrap iterations.
FIG 3
FIG 3
Alignment of partial 16S rRNA gene sequence (positions 180 to 227) for M. tuberculosis (1), the patient's novel NTM isolate (2), M. celatum type 1 (3), M. terrae strain (4), and M. marinum (5). The grayed sequence is the target for the commercial M. tuberculosis complex DNA probe (Gen-Probe) between positions 188 and 207 of the 16S rRNA sequence. Nucleotides highlighted in yellow are differences in the 16S rRNA sequence of the NTM species (2 to 5) from that of M. tuberculosis (1). Dashes indicate deletions.
FIG 4
FIG 4
Phylogenetic relationship of the novel NTM isolate (TBL 1200985) and related species of Mycobacterium, based on the concatenation of hsp65, rpoB, and secA1 sequences. All sequences used, except for that of the novel NTM, are type strains. Sequences were aligned with MUSCLE (17) and the tree constructed by the neighbor-joining method using the Tamura-Nei distance model. All steps were performed using Geneious software. Nocardia farcinica ATCC 3318T was used as an outgroup (GenBank accession no. AY756523, AB243742, and AY781800). Branch support is recorded at the nodes as a percentage of 100 bootstrap iterations.
See this image and copyright information in PMC

References

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