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. 2014 May;21(2):160-6.
doi: 10.4103/0971-6580.139797.

Bacterial Decolorization of Textile Azo Dye Acid Orange by Staphylococcus hominis RMLRT03

Rajat Pratap Singh  1 , Pradeep Kumar Singh  1 , Ram Lakhan Singh  1
Affiliations

Bacterial Decolorization of Textile Azo Dye Acid Orange by Staphylococcus hominis RMLRT03

Rajat Pratap Singh et al. Toxicol Int. 2014 May.

Abstract

A bacterial strain RMLRT03 with ability to decolorize textile dye Acid Orange dye was isolated from textile effluent contaminated soil of Tanda, Ambedkar Nagar, Uttar Pradesh (India). The decolorization studies were performed in Bushnell and Haas medium (BHM) amended with Acid Orange dye. The bacterial strain was identified as Staphylococcus hominis on the basis of 16S rDNA sequence. The bacterial strain exhibited good decolorization ability with glucose and yeast extract supplementation as cosubstrate in static conditions. The optimal condition for the decolorization of Acid Orange dye by Staphylococcus hominis RMLRT03 strain were at pH 7.0 and 35°C in 60 h of incubation. The bacterial strain could tolerate high concentrations of Acid Orange dye up to 600 mg l(-1). The high decolorizing activity under natural environmental conditions indicates that the bacterial strain has practical application in the treatment of dye containing wastewaters.

Keywords: 16S rDNA; BHM; cosubstrate; decolorization; textile dye.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Phylogenetic tree of the Staphylococcus hominis RMLRT03 strain based on 16S rDNA partial sequences
Figure 2
Figure 2
Effect of shaking and static conditions on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain
Figure 3
Figure 3
Effect of pH on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain under static condition
Figure 4
Figure 4
Effect of temperature on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain under static condition
Figure 5
Figure 5
Effect of different carbon source on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain under static condition
Figure 6
Figure 6
Effect of different nitrogen source on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain under static condition
Figure 7
Figure 7
Effect of initial dye concentration on decolorization of Acid Orange (100 mg l-1) by Staphylococcus hominis RMLRT03 strain under static condition
See this image and copyright information in PMC

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