Enhancement of the Arthus reaction and suppression of delayed type hypersensitivity (DTH) by pluronic F-68, a detergent frequently used to prepare perfluorocarbon emulsions

Abstract

The effect of a 20% w/v RM101 (perfluorobutyltetrahydrofuran) emulsion containing 5% w/v of the detergent Pluronic F-68 or 5% w/v Pluronic F-68 given alone on the Arthus reaction and on delayed type hypersensitivity (DTH) were evaluated in female A/J mice. The test substances were administered i.v. at 1% body weight at 0,4,7,14 and 28 days prior to the i.p. immunization with 10(7) sheep red blood cells (SRBC). The increase in footpad swelling at 4 h (Arthus reaction) and at 24 h (DTH) after elicitation with the s.c. administration of 10(8) SRBC into the left footpad was used to assess immune competence. Pluronic F-68 given alone enhanced the Arthus reaction only when administered on day 0 of immunization. Pluronic F-68 given alone, as well as the perfluorocarbon emulsion containing Pluronic F-68, suppressed the 24 h DTH for as long as 4 days prior to immunization. Nonemulsified perfluorocarbon, on the other hand, had no effect on either the Arthus reaction or on DTH. The immunostimulatory agent, levamisole, administered (10 mg/kg i.p.) 1.5-2 h prior to immunization with SRBC counteracted both the Arthus reaction and the DTH response produced by Pluronic F-68. The present data clearly demonstrate that the changes in Arthus reaction and the DTH response are due to the Pluronic F-68 used to emulsify the RM101 perfluorocarbon; the changes induced by the detergent in these two immune parameters probably involve separate mechanisms.