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. 2014 Dec;102(6):1692-9.e6.
doi: 10.1016/j.fertnstert.2014.08.021. Epub 2014 Sep 23.

Blastocentesis: a source of DNA for preimplantation genetic testing. Results from a pilot study

Luca Gianaroli  1 , M Cristina Magli  2 , Alessandra Pomante  2 , Anna M Crivello  2 , Giulia Cafueri  2 , Marzia Valerio  2 , Anna P Ferraretti  2
Affiliations

Blastocentesis: a source of DNA for preimplantation genetic testing. Results from a pilot study

Luca Gianaroli et al. Fertil Steril. 2014 Dec.

Erratum in

  • Fertil Steril. 2015 Aug;104(2):498

Abstract

Objective: To investigate the presence of DNA in blastocyst fluids (BFs) and to estimate whether the chromosomal status predicted by its analysis corresponds with the ploidy condition in trophectoderm (TE) cells, the whole embryo, and that predicted by polar bodies (PBs) or blastomeres.

Design: Prospective study.

Setting: In vitro fertilization unit.

Patient(s): Seventeen couples undergoing preimplantation genetic screening with the use of array comparative genomic hybridization on PBs (n = 12) or blastomeres (n = 5).

Intervention(s): BFs and TE cells were retrieved from 51 blastocysts for separate chromosomal analysis.

Main outcome measure(s): Presence of DNA in BFs and assessment of the corresponding chromosome condition; correlation with the results in TE cells and those predicted by the analysis done at earlier stages.

Result(s): DNA was detected in 39 BFs (76.5%). In 38 of 39 cases (97.4%) the ploidy condition of BFs was confirmed in TE cells, and the rate of concordance per single chromosome was 96.6% (904/936). In relation to the whole embryo, the ploidy condition corresponded in all cases with a per-chromosome concordance of 98.1%. The testing of PBs and blastomeres had 93.3% and 100% prediction of BF ploidy condition with a concordance per chromosome of 93.5% and 94%, respectively.

Conclusion(s): Blastocentesis could represent an alternative source of material for chromosomal testing, because the BF is highly predictive of the embryo ploidy condition and chromosome content. Our data confirm the relevance of the oocyte and of the early-cleavage embryo in determining the ploidy condition of the resulting blastocyst.

Keywords: Blastocyst; blastocele; blastomere; polar bodies; preimplantation genetic screening.

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