Research for the influence of telomerase inhibitors on myeloma cell and therapy

Abstract

This paper aims to study the effect of telomerase inhibitors zidovudine (AZT) on cell morphology,survival rate and telomerase activity of in-vitro culture myeloma cell line (U266).It has provided experiment basis for applying telomerase inhibitors in multiple myeloma treatment. The myeloma cell line in logarithmic phase (U266) could be divided into AZT group and negative control group, which are added with 100 μL AZT respectively and diluted into different concentrations with culture solution (1,10,100 and 1000 μmol-L⁻¹) of AZT, as well as culture solution of the same volume. After cultivating for 24, 48, 72 h, morphological changes of U266 cell is observed under optical microscope. Survival rate of cell is detected with MTT method, the change of telomerase activity is detected with TRAP-PCR-ELISA method. There is significant change in U266 cell morphology after AZT effect, their volume has been smaller and shrunken. Its shape has changed from short spindle to polygon or irregular shape, and broken away from the neighboring cells. Through comparing with control group, the survival rate of U266 cell in AZT group has decreased significantly (P<0.05), it represents time and concentration dependence. Median lethal concentration of AZT on U266 cell (IC₅₀) is 1000 μmol-L⁻¹.The drop in telomerase activity has decreased by 55.74% (P<0.05) through applying 1000 μmol-L AZT on U266 cell for 72 h. AZT has significant inhibiting effect on the proliferation of myeloma cell line, and could reduce the telomerase activity of myeloma cell.