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. 2015 Feb;18(2):195-201.
doi: 10.1089/jmf.2013.3137. Epub 2014 Sep 29.

Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus

Cüneyt Asım Aral  1 , Servet Kesim, Henry Greenwell, Mehmet Kara, Aysun Çetin, Birkan Yakan
Affiliations

Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus

Cüneyt Asım Aral et al. J Med Food. 2015 Feb.

Abstract

The aim of this study was to evaluate the efficacy of the anti-inflammatory effects of propolis on the systemic and local effects on experimental periodontitis and diabetes. Fifty-six Wistar rats were divided into seven groups: (1) negative-control (NC), (2) periodontitis (P), (3) diabetes (D), (4) diabetes+periodontitis (DP), (5) periodontitis+propolis (P-Pro), (6) diabetes+propolis (D-Pro), and (7) diabetes+periodontitis+propolis (DP-Pro). Periodontitis was induced by ligature placement and diabetes was induced by streptozotocin injection. Propolis (Pro) was administrated by oral gavage (100 mg/kg/day). On day 21, plasma was obtained for analysis and alveolar bone level was evaluated using histomorphometric analysis. Compared to NC the final blood glucose levels for D-Pro was not significantly different (P=.052), however, D, DP, and DP-Pro were significantly different. There were no statistically significant differences in blood glucose concentrations between P and P-Pro, between D and D-Pro, and between DP and DP-Pro. All groups showed significantly more alveolar bone loss compared with NC. A significant difference in bone loss was found between P and P-Pro, and DP and DP-Pro, however there was no difference between D and D-Pro. Plasma interleukin 1beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and matrix metalloproteinase-8 (MMP-8) levels were not significantly different among groups. In conclusion, propolis reduced fasting blood glucose levels in diabetes. In addition, propolis might be beneficial as an adjunct treatment of diabetes associated periodontitis and periodontitis without diabetes.

Keywords: diabetes mellitus; interleukin 1beta; matrix metalloproteinase-8; periodontitis; plasma; propolis; tumor necrosis factor-alpha.

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Figures

<b>FIG. 1.</b>
FIG. 1.
Photographs illustrating alveolar bone status between the first and second molars (magnification of ×4). Arrows indicate alveolar bone crest level. Bars define metric magnification. Masson's trichrome staining was used. (A) Negative-control (NC) group (B) periodontitis (P) group (C) diabetes (D) group (D) diabetes+periodontitis group (E) periodontitis+propolis (P-Pro) 100 mg/kg group (F) diabetes+propolis (D-Pro) 100 mg/kg group (G) diabetes+periodontitis+propolis 100 mg/kg group.
<b>FIG. 2.</b>
FIG. 2.
Final blood glucose of groups. Bars express mean±standard deviation. * Comparisons were made between NC and other groups. D, DP, and DP-Pro significantly differed from NC (P<.05). † D was significantly greater compared with P (P<.05). ‡ DP was significantly greater compared with P (P<.05). Color images available online at www.liebertpub.com/jmf
<b>FIG. 3.</b>
FIG. 3.
Cementoenamel junction to the alveolar bone crest (CEJ-ABC) measurements of groups. Bars express mean±standard deviation. * Comparisons were made NC versus other groups. All groups significantly differed from NC (P<.05). † P was significantly greater compared with D (P<.05). ‡ DP was significantly greater compared with D (P<.05). § P was significantly greater compared with P-Pro (P<.05). ∥ DP was significantly greater compared with DP-Pro (P<.05). Color images available online at www.liebertpub.com/jmf
See this image and copyright information in PMC

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