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. 1978 Mar;83(3):893-903.
doi: 10.1093/oxfordjournals.jbchem.a131987.

Purification and characterization of lysozyme produced by Streptomyces erythraeus

Free article

Purification and characterization of lysozyme produced by Streptomyces erythraeus

T Morita et al. J Biochem. 1978 Mar.
Free article

Abstract

A species of lysozyme (SE lysozyme) was purified from culture filtrate of Streptomyces erythraeus. The enzyme has a molecular weight of 18,500 as determined by ultracentrifugation. Its isoelectric point is 9.5, and it shows optimal activity at pH 4.0 with an optimal ionic strength of 0.1. Investigation of the substrate specificity showed SE lysozyme to be an N-acetyl-muramidase. The simplest product in the digest of cell walls of Micrococcus lysodeikticus was identified as a disaccharide, [GlcNAcbeta(1 leads to 4) MurNAc]. While S. aureus as well as M. lysodeikticus was lysed by this lysozyme, chitin and its derivatives were not.

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